4/30/2023 0 Comments Install qq international![]() ![]() From mini fermentation models such as Mini Bio with 32 bioreactors or MiPro using 96-deep well plates to bigger models similar to the mono-compartmental colonic ARCOL (Artificial Colon) or the 4 fermenters TIM-2 (TNO intestinal model 2) or the upper colonic PolyFermS, other more complex models exist. In contrast, dynamic models seek to mimic continuous fermentations by a constant addition of nutrients to the system. The first, also called batch fermentation models, such as CoMiniGut, are mainly used for preliminary investigations, to simulate the fermentations usually observed in the distal part of the colon for a short period of time and using a consensus protocol (InfoGest). Regarding in vitro colon fermentation models, 2 types of systems exist: the static and the dynamic models. In vivo and ex vivo models (using humans, animals or organs biopsy) are limited mainly because of ethical restrictions. To assess the efficacy of new phages, and their effect on human intestinal microbiota, several in vitro, in vivo, or ex vivo models exist. Even if further in vitro studies are needed to assess the efficacy of this phage against its bacterial host within the human intestinal ecosystem, the phage ULIP33 exerted no significant change on the global colonic microbiota. Diversity (α and β), the relative abundance of bacteria, and qPCR analysis targeting different genera of interest showed no significant variation following phage administration. The concentration of short chain fatty acids in the colons showed good colonization of the bioreactors by the microbiota and no significant effect related to the phage treatment. ![]() After stabilization of the system, the phage was inoculated for 7 days and its persistence in the different colons was studied until its disappearance from the system. To that end, the effect of the phage vB_KpnP_K1-ULIP33, whose host is the hypervirulent Klebsiella pneumoniae SA12 (ST23 and capsular type K1), was assessed on intestinal microbiota, using an in vitro model: the SHIME ® system (Simulator of the Human Intestinal Microbial Ecosystem). ![]() New control methods are needed to counter antimicrobial resistances and the use of bacteriophages as an alternative treatment seems promising. ![]()
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